1 Scope
This standard specifies the detection of clostridium botulinum in foods by PCR.
This standard is applicable to the detection of Types A, B, E and F clostridium botulinum in foods.
2 Normative References
The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition of the normative document referred to applies.
GB/T 4789.12 Microbiological Examination of Food Hygiene - Examination of Clostridium Botulinum and Botulinus Toxi
GB/T 6682 Water for Analytical Laboratory Use - Specification and Test Methods
GB 19489 Laboratories - General Requirements for Biosafety
GB/T 27403 Criterion on Quality Control of Laboratories - Molecular Biological Testing of Food
SN/T 1193 General Requirements for the Laboratories for Gene Detection and Identification
3 Terms, Definitions and Abbreviations
For the purposes of this standard, the following terms, definitions and abbreviations apply.
Foreword i
1 Scope
2 Normative References
3 Terms, Definitions and Abbreviations
4 Biosafety Measures
5 Contamination Prevention Measures
6 Method Summary
7 Equipment and Materials
8 Culture Media and Reagents
9 Inspection Procedures
10 Inspection Steps
11 PCR Result Judgment
12 Confirmatory Test
13 Result Report
Annex A (Normative) Preparation of Culture Media and Reagents
Annex B (Normative) Table for Reference of Detection Method of Clostridium Botulinum by PCR
