GB/T 45422-2025 Chemicals―Surface water-sediment Misgurnus anguillicaudatus toxicit English, Anglais, Englisch, Inglés, えいご
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ICS13.300
CCS A 80
National Standards of the People's Republic of China
GB/T 45422-2025
Chemicals - Surface water - sediment Misgurnus anguillicaudatus toxicity test
化学品 地表水系统 沉积物中泥鳅毒性试验
(English Translation)
Issue date: 2025-02-28 Implementation date: 2026-09-01
Issued by the State Administration for Market Regulation
the Standardization Administration of the People's Republic of China
Contents
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Test principle
5 Test substance information
6 Instruments and equipment
7 Test system
8 Test procedure
9 Quality assurance and quality control
10 Data and reporting
Annex A (Informative) Biological Characteristics of Loach
Annex B (Normative) Chemical Characterization of Test Water
Annex C (Informative) Composition of Prepared Sediment
Bibliography
Chemicals - Surface water - sediment Misgurnus anguillicaudatus toxicity test
1 Scope
This document describes chemical surface water systems﹘Test principle, test substance information, instruments and equipment, test system, test procedure, quality assurance and quality control, data and reports of loach toxicity test in sediments.
This document is suitable for testing the toxic effects of chemicals on the benthic loach in surface water environments and is not suitable for volatile substances testing.
2 Normative references
The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, only the version corresponding to the date is applicable to this document for the referenced documents with a date; the latest version of the undated referenced document (including all amendments) is applicable to this document.
GB/T 5750.4 Standard test methods for drinking water - Part 4: Sensory properties and physical indicators
GB/T 11901 Water Determination of suspended solids Gravimetric method
GB 17378.5 Code for marine monitoring - Part 5: analysis of sediments
HJ 494 Technical guidance on water quality sampling
HJ 501 Water quality Determination of total organic carbon Combustion oxidation - Non-dispersive infrared absorption method
HJ 586-2010 Determination of free chlorine and total chlorine in water quality N, N-Diethyl-1, 4-Phenylenediamine spectrophotometry
HJ 1257 Chemical substances environmental management Chemical substance testing terminology
3 Terms and definitions
The terms and definitions defined in HJ 1257 and the following apply to this document.
3.1
overlying water
water on top of sediment in test vessel
3.2
interstitial water or pore water
water occupying space inside sediment or soil particles
3.3
formulated sediment
mixture consisting of multiple materials for simulating natural sediment compositions
Note: Also known as reconstructed or synthetic deposits.
3.4
spiked sediment
deposits with added test substance
3.5
median lethal concentration; LC50
concentration of the target test chemical that causes the death of half of a group of test organisms
4 Test principle
Domesticated loaches with the same size (4 cm ~ 6 cm) and similar physiological status were selected and exposed to sediments containing a series of concentrations of test substances - water system, the toxicity of the test substance to loach was determined. The test substance was added to the sediment and pairs were set without the addition of the test substance Photo. After the concentration of the test substance reached equilibrium between sediment and water, loach was added and exposed for 96 h or 21 d. The death, balance damage, gill bleeding and other poisoning conditions of loach were recorded, and the 10% lethal concentration (LC10), LC50 (LC50) and half effect concentration (EC50) and other parameters.
5 Test substance information
Before the test, the following information of the test substance should be mastered:
a) Structural formula;
b) Purity;
c) Solubility in water;
d) Vapor pressure;
e) Dissociation constant (pKa);
f) N-octanol﹘Water partition coefficient (Kow);
g) Stability in water and sediment;
h) Methods for quantitative analysis of test substances in sediment, overlying water, pore water or interstitial water;
i) Rapid biodegradation test results.
6 Instruments and equipment
Test vessels and other appliances in direct contact with the test system shall be made of glass or other chemically inert materials.
Other instruments and equipment include pH meter, dissolved oxygen meter, hardness tester, conductivity meter, organic carbon analyzer, aeration device, and other related equipment required for chemical analysis.
7 Test system
7.1 Test organisms
7.1.1 Selection of test organisms
The test organism is Misgurnus anguillicaudatus, that is, green loach, belonging to the order Cypriniformes and the family Loachidae. The loaches used in the test should be taken from populations domesticated in the same laboratory environment. The environmental conditions during domestication of loach should be consistent with those during experiment. The biological characteristics of loach are shown in Annex A.
7.1.2 Domestication of test organisms
Loach is domesticated in an inert material container with a water depth of 5cm ~ 7cm. Test water after being aerated for 48 hours is added into the container, the water temperature is 18 ℃ ~ 25 ℃, the pH value of the water body is 6.0 ~ 9.0, the dissolved oxygen is not less than 60% of the air saturation value at the used temperature, and the illumination is 12h ~ 16h every day. Feed fish food at least once a week, and the feeding amount is 4% ~ 8% of the total weight of loach, and remove feces and residual bait in time. During the domestication process, the test water was added in time to keep the water quantity constant, and the water was changed once to twice a week. Loach domesticated for 2 weeks or more and mortality less than 10% can be used in the experiment.
7.1.3 Selection of test organisms
The loaches with the same body color, healthy body length of 4cm ~ 6cm were randomly selected from the successfully domesticated loach populations with the standard mortality rate to carry out the experiment. The loaches used in the same batch of experiments were from healthy individuals of the same population. Before the test, loach was fasted for 24 hours for intestinal cleansing.
