GB/T 14926.45-2026 Laboratory animal—Method for examination of Brucella spp. English, Anglais, Englisch, Inglés, えいご
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ICS 13.220.10
CCS H 57
National Standard of the People's Republic of China
GB/T 14926.45-2026
Replaces GB/T 14926.45-2001
Laboratory animal - Method for examination of Brucella spp.
实验动物 布鲁氏菌检测方法
Issue date: 2026-01-28 Implementation date: 2027-02-01
Issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
the Standardization Administration of the People's Republic of China
Contents
Foreword
Introduction
1 Scope
2 Normative References
3 Terms and Definitions
4 Clinical Symptoms and Pathological Changes
5 Biosafety Measures
6 Detection Methods
7 Result Reporting
Laboratory Animals — Detection Method for Brucella spp.
1 Scope
This document describes detection methods for Brucella spp. in laboratory animals.
This document applies to the detection of Brucella spp. in laboratory animals such as dogs, pigs, cattle, and sheep, or in experimental inocula and environmental samples from laboratory animal facilities.
2 Normative References
The following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 18646 Diagnostic techniques for animal brucellosis
GB 19489 Laboratory – General requirements for biosafety
NY/T 541 Technical specification for collection, preservation and transportation of veterinary diagnostic samples
SN/T 1088 Technical specification for quarantine of brucellosis
WS 269 Diagnosis for brucellosis
3 Terms and Definitions
No terms and definitions are required for this document.
4 Clinical Symptoms and Pathological Changes
Implement according to SN/T 1088.
5 Biosafety Measures
Experimental operations and handling shall be carried out according to the provisions of GB 19489. Identification of Brucella pathogens and serological tests shall be performed by qualified personnel. All cultures and waste shall be disposed of after autoclaving. Pathogen isolation and culture work shall be conducted in a biosafety level 3 laboratory.
6 Detection Methods
6.1 General Requirements
Screening tests shall be performed first. One or more screening tests may be selected. Animals positive in screening tests shall undergo confirmatory tests. One or more confirmatory tests may be selected. When necessary, two or more methods may be combined for testing to avoid missed detection.
6.2 Sample Collection, Processing, Preservation and Transportation
Collect, preserve, and transport whole blood and serum according to NY/T 541. Collect, process, preserve, and transport tissue samples, swab samples, and liquid samples (vaginal secretions, amniotic fluid, synovial fluid, semen, etc.) according to GB/T 18646.
6.3 Screening Test — Rose Bengal Plate Test (RBT)
6.3.1 Reagents and Materials
6.3.1.1 Rough and smooth Brucella Rose Bengal plate test antigen.
6.3.1.2 Rough and smooth Brucella standard positive serum.
6.3.1.3 Brucella standard negative serum.
6.3.1.4 Timer.
6.3.1.5 Pipette tips.
6.3.1.6 Toothpicks or mixing sticks.
6.3.1.7 Clean glass plate.
6.3.2 Instruments and Equipment
6.3.2.1 Centrifuge (centrifugal force above 1000g).
6.3.2.2 Class II biosafety cabinet.
6.3.2.3 Adjustable micropipette (10 μL – 100 μL).
6.3.3 Sample
Serum.
6.3.4 Test Procedure
Perform the Rose Bengal plate test according to GB/T 18646.
6.3.5 Result Interpretation
6.3.5.1 The test is valid only when the standard negative serum shows no agglutination and the positive serum shows agglutination; only then can the test sera be interpreted.
6.3.5.2 Visible agglutination is interpreted as positive (+). No agglutination, with the serum-antigen mixture remaining uniformly pink, is interpreted as negative (-).
6.4 Screening Test — Colloidal Gold Immunochromatographic Assay (GICA)
6.4.1 Reagents and Materials
6.4.1.1 Rough and smooth Brucella antibody detection cards (colloidal gold method).
6.4.1.2 Rough and smooth Brucella standard positive serum.
6.4.1.3 Brucella standard negative serum.
6.4.1.4 Timer.
6.4.1.5 Pipette tips.
6.4.2 Instruments and Equipment
6.4.2.1 Centrifuge (centrifugal force above 1000g).
6.4.2.2 Class II biosafety cabinet.
6.4.2.3 Adjustable micropipette (10 μL – 100 μL).
6.4.3 Sample
Serum or plasma.
6.4.4 Test Procedure
Perform the immunochromatographic assay according to GB/T 18646.
6.4.5 Result Interpretation
6.4.5.1 Validity condition: The test result is valid only when a reaction indicator line appears at the position marked "C" on the test strip. Otherwise, the test result is invalid.
6.4.5.2 Positive: Two reaction indicator lines appear, one at the position marked "C" and another at the position marked "T", interpreted as positive.
6.4.5.3 Negative: Only one reaction indicator line appears, at the position marked "C", and no line appears at the position marked "T", interpreted as negative.
Note: Refer to the kit instructions for operation and result interpretation.
6.5 Screening Test — Indirect Enzyme-Linked Immunosorbent Assay (iELISA)
6.5.1 Reagents and Materials
6.5.1.1 Brucella indirect enzyme-linked immunosorbent assay (iELISA) detection kit.
6.5.1.2 Rough and smooth Brucella standard positive serum.
6.5.1.3 Brucella standard negative serum.
6.5.1.4 Pipette tips.
6.5.2 Instruments and Equipment
6.5.2.1 Incubator (36°C ± 1°C).
6.5.2.2 Centrifuge (centrifugal force above 1000g).
6.5.2.3 Microplate reader (with 405 nm, 450 nm, 492 nm filters).
6.5.2.4 Class II biosafety cabinet.
6.5.2.5 Adjustable micropipettes (0.5 μL – 10 μL, 10 μL – 100 μL, 100 μL – 1000 μL).
6.5.3 Sample
Serum or plasma.
6.5.4 Test Procedure
Perform the indirect enzyme-linked immunosorbent assay according to GB/T 18646.
6.5.5 Result Interpretation
When the standard positive and negative serum controls are valid, interpret the results according to the kit instructions.
