GB/T 45774.1-2025 Quantitative determination of antibacterial activity of ceramic tile surfaces - Test methods - Part 1: Ceramic tile surfaces with incorporated antibacterial agents
1 Scope
This document specifies test methods for evaluating the antibacterial activity of glazed and unglazed ceramic tile surfaces with incorporated antibacterial agents.
Secondary effects on ceramic tile surfaces due to antibacterial treatments, such as changes in chemical resistance, stain resistance or small colour differences, are not covered by this document. For chemical resistance refer to ISO 10545-13, for stain resistance refer to ISO 10545-14 and for colour differences refer to ISO 10545-16.
Any results obtained with this document will always refer to this document and the conditions used. Results obtained with this document indicate antibacterial activity under the specified experimental conditions used herein, and do not reflect activity under other circumstances where a variety of factors, such as temperature, humidity, different bacterial species and nutrient conditions, are considered.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
antibacterial
condition where growth of bacteria on the surfaces of product is suppressed or describing the effect of an agent which suppresses the growth of bacteria on the surface of products
3.2
antibacterial agent
agent that kills or inhibits growth of bacteria on the surfaces of products by the use of an antibacterial (3.1) surface treatment or a compounded agent
3.3
antibacterial activity
difference in the logarithm of the viable cell count found on an antibacterial-treated product and an untreated control after inoculation with and culture of bacteria
4 Materials
4.1 Bacterial strains
This test method was developed with the following bacteria:
a) Escherichia coli;
b) Staphylococcus aureus.
The test method shall be conducted using either of the above-mentioned bacteria. The bacterial strains to be used in the test are listed in Table 1. Other bacterial species may be tested with this test method, details of the species used, culture conditions and testing process shall be described in detail in the test report.
Contents
Foreword i
Introduction ii
1 Scope
2 Normative references
3 Terms and definitions
4 Materials
4.1 Bacterial strains
4.2 Culture media and reagents
4.2.1 Non-ionic surfactant
4.2.2 Nutrient broth
4.2.3 1/500 nutrient broth
4.2.4 Nutrient agar
4.2.5 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP)
4.2.6 Phosphate buffer saline (PBS)
5 Test specimen
5.1 Size
5.2 Control specimen
5.3 Preconditioning
5.4 Number of test specimens
6 Procedure
6.1 Preparation of test inoculum
6.2 Cover film
6.3 Inoculation of test specimen
6.4 Recovery of bacteria from the untreated control specimens at time t=0h (6.4)
6.5 Culture period
6.6 Recovery of bacteria from post culture test specimens
6.7 Counting of viable bacteria in test specimen washout solution
7 Validation and calculation
7.1 General
7.2 Criteria for a valid test
7.3 Calculation of antibacterial activity
8 Test report
Bibliography
