1 Scope
This standard specifies the high performance liquid chromatography for the determination of neotame content in foods.
This standard is applicable to the determination of neotame in foods such as beverage, preserved fruit, pastry, roasted seeds and nuts, pickled vegetable, candy, jam, jelly and compound flavouring.
2 Principle
Specimen is determined with high performance liquid chromatograph after it is extracted with mixed extracting solution and purified with solid-phase extraction column. Qualitative analysis is conducted by retention time and quantitative analysis is conducted by external standard method of peak area.
3 Reagents and Materials
Unless otherwise specified, the reagents adopted in this method are all analytically pure, and the water is Grade 1 water specified in GB/T 6682.
Foreword i
1 Scope
2 Principle
3 Reagents and Materials
4 Instruments and Apparatus
5 Analytical Procedures
6 Expression of Analysis Results
7 Precision
8 Others
Appendix A Chromatogram