GB 7300.506-2025 Feed additives—Part 5：Live microorganisms—Lactobacillus delbrueckii subsp. lactis English, Anglais, Englisch, Inglés, えいご
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ICS
CCS H
National Standard of the People's Republic of China
GB 7300.506-2025
Feed additives - Part 5: Live microorganisms - Lactobacillus delbrueckii subsp.lactis
饲料添加剂 第5部分：微生物 德式乳杆菌乳酸亚种
Issue date: 2025-12-31 Implementation date: 2027-01-01
Issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
the Standardization Administration of the People's Republic of China
Contents
Foreword
Introduction
1 Scope
2 Normative References
3 Terms and Definitions
4 Raw Materials and Strain
5 Technical Requirements
6 Sampling
7 Test Methods
8 Inspection Rules
9 Labeling, Packaging, Transportation, Storage and Shelf Life
Introduction
Feed additives refer to substances added in small or trace amounts during the processing, manufacturing, and use of feed, including nutritional feed additives and general feed additives. For ease of use, and according to product type, GB 7300 "Feed Additives" is divided into the following 13 major categories:
— Amino acids, amino acid salts and analogues;
— Vitamins and vitamin-like substances;
— Mineral elements and their complexes (chelates);
— Enzyme preparations;
— Microorganisms;
— Non-protein nitrogen;
— Antioxidants;
— Preservatives, mold inhibitors and acidity regulators;
— Coloring agents;
— Flavoring and appetite-stimulating substances;
— Binders, anti-caking agents, stabilizers and emulsifiers;
— Polysaccharides and oligosaccharides;
— Others.
The product Lactobacillus delbrueckii subsp. lactis covered by this document belongs to the fifth major category: Microorganisms. As Lactobacillus delbrueckii subsp. lactis is the sixth product standard published under this major category, this document is assigned the number GB 7300.506 and serves as Part 506 of GB 7300.
Feed Additives — Part 5: Microorganisms — Lactobacillus delbrueckii subsp. lactis
1 Scope
This document specifies the technical requirements, sampling, inspection rules, labeling, packaging, transportation, storage, and shelf life of the feed additive Lactobacillus delbrueckii subsp. lactis. It also describes the corresponding test methods.
This document applies to solid feed additive products obtained using Lactobacillus delbrueckii subsp. lactis as the strain, through processes such as liquid fermentation, separation, with or without the addition of a carrier, and drying.
2 Normative References
The contents of the following documents constitute essential provisions of this document through normative reference. For dated references, only the edition corresponding to the date applies. For undated references, the latest edition (including any amendments) applies.
GB/T 5917.1 Determination of Feed Particle Size — Sieving Method Using Two Sieves
GB/T 6435 Determination of Moisture in Feeds
GB/T 6682 Water for Analytical Laboratory Use — Specification and Test Methods
GB/T 8170 Rules of Rounding Off for Numerical Values & Expression and Judgment of Limiting Values
GB 10648 Feed Labeling
GB/T 13079 Determination of Total Arsenic in Feeds
GB/T 13080 Determination of Lead in Feeds — Atomic Absorption Spectrometry
GB/T 13081 Determination of Mercury in Feeds
GB/T 13082 Determination of Cadmium in Feeds
GB/T 13091 Determination of Salmonella in Feeds
GB/T 13092 Determination of Total Mold Count in Feeds
GB/T 14699 Feeding Stuffs — Sampling
GB/T 18869 Determination of Coliforms in Feeds
GB/T 28716 Determination of Zearalenone in Feeds — Immunoaffinity Column Cleanup-High Performance Liquid Chromatography
GB/T 30956 Determination of Deoxynivalenol in Feeds — Immunoaffinity Column Cleanup-High Performance Liquid Chromatography
GB/T 42959 Feed Microbiological Testing — Sampling
NY/T 2071 Determination of Aflatoxins, Zearalenone and T-2 Toxin in Feeds — Liquid Chromatography-Tandem Mass Spectrometry
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Lactobacillus delbrueckii subsp. lactis
Belongs to the domain Bacteria, phylum Firmicutes, class Bacilli, order Lactobacillales, family Lactobacillaceae, genus Lactobacillus.
Note: Gram-positive rods, cells are long rods, tending to be linear; non-motile. Anaerobic or facultatively anaerobic, chemoheterotrophic, homofermentative, producing D-lactic acid.
4 Raw Materials and Strain
4.1 Raw Materials
Shall be sourced from the varieties listed in the Feed Ingredients Catalog and/or the Feed Additive Varieties Catalog.
4.2 Strain
The strain shall be Lactobacillus delbrueckii subsp. lactis.
5 Technical Requirements
5.1 Appearance and Properties
Powder or granules, uniform in form and color, free from caking, mold, and abnormal odor.
5.2 Identification of Lactobacillus delbrueckii subsp. lactis
Shall comply with the morphological characteristics, physiological and biochemical characteristics, and molecular biology results described in Annex A.
5.3 Quality Indicators
Shall comply with the requirements specified in Table 1.
5.4 Hygiene Indicators
Shall comply with the requirements specified in Table 2.
6 Sampling
Sampling for microbiological testing purposes shall be carried out according to GB/T 42959. Sampling for other indicator testing purposes shall be carried out according to GB/T 14699.
7 Test Methods
7.1 Appearance and Properties
Place an appropriate amount of the sample on clean white paper. Observe its form, color, and the presence of foreign matter, and smell its odor under natural light.
7.2 Identification of Lactobacillus delbrueckii subsp. lactis
Determine according to the method specified in Annex A.
7.3 Viable Count of Lactobacillus delbrueckii subsp. lactis
Determine according to the method specified in Annex B.
7.4 Moisture
Determine according to the method specified in GB/T 6435.
7.5 Particle Size
Determine according to the method specified in GB/T 5917.1.
7.6 Total Arsenic (as As)
Determine according to the method specified in GB/T 13079.
7.7 Lead
Determine according to the method specified in GB/T 13080.
7.8 Mercury
Determine according to the method specified in GB/T 13081.
7.9 Cadmium
Determine according to the method specified in GB/T 13082.
7.10 Aflatoxin B₁
Determine according to the method specified in NY/T 2071.
7.11 Deoxynivalenol (Vomitoxin)
