GB 7300.208-2025 Feed additives—Part 2: Vitamins,provitamins and chemically well-defined substances having similar effects—Calcium L-Ascorbate English, Anglais, Englisch, Inglés, えいご
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ICS
CCS H
National Standard of the People's Republic of China
GB 7300.208-2025
Replaces GB 34463-2017
Feed additives - Part 2: Vitamins, provitamins and chemically well-defined substances having similar effects - Calcium L-Ascorbate
饲料添加剂 第2部分：维生素及类维生素 L-抗坏血酸钙
Issue date: 2025-12-31 Implementation date: 2027-01-01
Issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
the Standardization Administration of the People's Republic of China
Contents
Foreword
Introduction
1 Scope
2 Normative References
3 Terms and Definitions
4 Chemical Name, Molecular Formula, Relative Molecular Mass and Structural Formula
5 Technical Requirements
6 Sampling
7 Test Methods
8 Inspection Rules
9 Labeling, Packaging, Transportation, Storage and Shelf Life
Introduction
Feed additives refer to substances added in small or trace amounts during the processing, manufacturing, and use of feed, including nutritional feed additives and general feed additives. For ease of use, and according to product type, GB 7300 "Feed Additives" is divided into the following 13 parts:
— Part 1: Amino acids, amino acid salts, and analogues;
— Part 2: Vitamins and vitamin-like substances;
— Part 3: Mineral elements and their complexes (chelates);
— Part 4: Enzyme preparations;
— Part 5: Microorganisms;
— Part 6: Non-protein nitrogen;
— Part 7: Antioxidants;
— Part 8: Preservatives, mold inhibitors, and acidity regulators;
— Part 9: Coloring agents;
— Part 10: Flavoring and appetite-stimulating substances;
— Part 11: Binders, anti-caking agents, stabilizers, and emulsifiers;
— Part 12: Polysaccharides and oligosaccharides;
— Part 13: Others.
The product choline chloride covered by this document belongs to Part 2: Vitamins and vitamin-like substances. As choline chloride is the sixth product standard published under this major category, this document is assigned the number GB 7300.206 and serves as Part 206 of GB 7300.
Feed Additives — Part 2: Vitamins and Vitamin-Like Substances — Calcium L-Ascorbate
1 Scope
This document provides the chemical name, molecular formula, relative molecular mass and structural formula of calcium L-ascorbate, and specifies the technical requirements, inspection rules, labeling, packaging, transportation, storage, and shelf life of the feed additive calcium L-ascorbate. It also describes the sampling and test methods.
This document applies to the feed additive calcium L-ascorbate obtained by chemical synthesis using L-ascorbic acid and calcium salts as raw materials.
2 Normative References
The contents of the following documents constitute essential provisions of this document through normative reference. For dated references, only the edition corresponding to the date applies. For undated references, the latest edition (including any amendments) applies.
GB/T 601 Chemical Reagents — Preparations of Standard Volumetric Solutions
GB/T 613 Chemical Reagents — General Method for the Determination of Specific Optical Rotation
GB/T 6435 Determination of Moisture in Feeds
GB/T 6682 Water for Analytical Laboratory Use — Specification and Test Methods
GB/T 8170 Rules of Rounding Off for Numerical Values & Expression and Judgment of Limiting Values
GB/T 9724 Chemical Reagents — General Rules for the Determination of pH
GB 10648 Feed Labeling
GB/T 13079 Determination of Total Arsenic in Feeds
GB/T 13080 Determination of Lead in Feeds — Atomic Absorption Spectrometry
GB/T 13083 Determination of Fluorine in Feeds — Ion Selective Electrode Method
GB/T 14699 Feeding Stuffs — Sampling
3 Terms and Definitions
No terms and definitions are stipulated in this document.
4 Chemical Name, Molecular Formula, Relative Molecular Mass and Structural Formula
4.1 Chemical name: Calcium L-ascorbate.
4.2 Molecular formula: C₁₂H₁₄CaO₁₂·2H₂O.
4.3 Relative molecular mass: 426.35 (based on the 2022 international relative atomic masses).
4.4 Structural formula: Shown in Figure 1.
5 Technical Requirements
5.1 Appearance and Properties
Shall be white or pale yellow crystalline powder, odorless, soluble in water.
5.2 Identification
5.2.1 Chemical identification: Shall comply with the identification reactions for calcium ion and ascorbate radical.
5.2.2 Infrared identification: The infrared spectrum shall be consistent with that of the calcium L-ascorbate reference standard.
5.3 Physicochemical Indicators
Shall comply with the requirements specified in Table 1.
5.4 Hygiene Indicators
Shall comply with the requirements specified in Table 2.
6 Sampling
Carry out according to GB/T 14699.
7 Test Methods
Warning: Some reagents used in the test methods are corrosive. Exercise caution during operation and take appropriate safety and protective measures.
7.1 General Provisions
Unless otherwise specified, all reagents used shall be of analytical grade; all standard volumetric solutions shall be prepared and standardized according to GB/T 601; water used in tests shall be Grade 3 water as specified in GB/T 6682.
7.2 Appearance and Properties
7.2.1 Place an appropriate amount of the sample on a clean, dry white porcelain plate. Observe its color, form, and smell its odor under natural light.
7.2.2 Weigh 1 g of the sample, dissolve in 10 ml of water, shake for 30 seconds. It should dissolve completely, forming a colorless, transparent solution.
7.3 Identification
7.3.1 Reagents or Solutions
7.3.1.1 Potassium bromide: spectroscopic grade.
7.3.1.2 Ammonia solution: Measure 400 ml of ammonia water, dilute with water to 1000 ml, and mix well.
7.3.1.3 Hydrochloric acid solution: Measure 234 ml of hydrochloric acid, slowly add to water and dilute to 1000 ml, mix well.
7.3.1.4 Ammonium oxalate solution: Weigh 3.5 g of ammonium oxalate, dissolve in water and dilute to 100 ml, mix well.
7.3.1.5 Sodium hydroxide solution (0.05 mol/L): Weigh 110 g of sodium hydroxide, dissolve in 100 ml of carbon dioxide-free water, shake well, pour into a polyethylene container, and seal until the solution becomes clear. Using a plastic pipette, transfer 2.7 ml of the supernatant, dilute to 1000 ml with carbon dioxide-free water, and mix well.
7.3.1.6 Sodium 2,6-dichloroindophenol solution: Weigh 0.1 g of sodium 2,6-dichloroindophenol into a brown bottle, dissolve in and dilute to 100 ml with water, filter. Store at 2°C to 8°C, validity period is one week.
7.3.1.7 Methyl red indicator: Weigh 0.1 g of methyl red, add 7.4 ml of sodium hydroxide solution (7.3.1.5) to dissolve, dilute to 200 ml with water, and mix well.
7.3.2 Apparatus
7.3.2.1 Analytical balance: accuracy 0.01 g and 0.1 mg.
7.3.2.2 Infrared spectrophotometer: resolution ≤ 4.0 cm⁻¹.
7.3.3 Test Procedure
7.3.3.1 Chemical Identification
7.3.3.1.1 Weigh 1 g of the sample, add 10 ml of water to dissolve, add 2 drops of methyl red indicator (7.3.1.7). Dropwise add ammonia solution (7.3.1.2) until the solution turns yellow, then dropwise add hydrochloric acid solution (7.3.1.3) until the solution changes from yellow to red. Add 2 ml of ammonium oxalate solution (7.3.1.4), filter to obtain the ator.
