GB 7300.207-2025 Feed additives—Part 2: Vitamins, provitamins and chemically well-defined substances having similar effects—Nicotinamide English, Anglais, Englisch, Inglés, えいご
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ICS
CCS H
National Standard of the People's Republic of China
GB 7300.207-2025
Replaces GB 7301-2017
Feed additives - Part 2: Vitamins, provitamins and chemically well-defined substances having similar effects - Nicotinamide
饲料添加剂 第2部分：维生素及类维生素 烟酰胺
Issue date: 2025-12-31 Implementation date: 2027-01-01
Issued by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
the Standardization Administration of the People's Republic of China
Contents
Foreword
Introduction
1 Scope
2 Normative References
3 Terms and Definitions
4 Chemical Name, Molecular Formula, Relative Molecular Mass and Structural Formula
5 Raw Materials
6 Technical Requirements
7 Sampling
8 Test Methods
9 Inspection Rules
10 Labeling, Packaging, Transportation, Storage and Shelf Life
Introduction
Feed additives refer to substances added in small or trace amounts during the processing, manufacturing, and use of feed, including nutritional feed additives and general feed additives. For ease of use, and according to product type, GB 7300 "Feed Additives" is divided into the following 13 parts:
— Part 1: Amino acids, amino acid salts, and analogues;
— Part 2: Vitamins and vitamin-like substances;
— Part 3: Mineral elements and their complexes (chelates);
— Part 4: Enzyme preparations;
— Part 5: Microorganisms;
— Part 6: Non-protein nitrogen;
— Part 7: Antioxidants;
— Part 8: Preservatives, mold inhibitors, and acidity regulators;
— Part 9: Coloring agents;
— Part 10: Flavoring and appetite-stimulating substances;
— Part 11: Binders, anti-caking agents, stabilizers, and emulsifiers;
— Part 12: Polysaccharides and oligosaccharides;
— Part 13: Others.
The product choline chloride covered by this document belongs to Part 2: Vitamins and vitamin-like substances. As choline chloride is the sixth product standard published under this major category, this document is assigned the number GB 7300.206 and serves as Part 206 of GB 7300.
Feed Additives — Part 2: Vitamins and Vitamin-Like Substances — Nicotinamide
1 Scope
This document provides the chemical name, molecular formula, relative molecular mass and structural formula of nicotinamide, and specifies the technical requirements, inspection rules, labeling, packaging, transportation, storage, and shelf life of the feed additive nicotinamide. It also describes the sampling and test methods.
This document applies to the feed additive nicotinamide produced by chemical synthesis from 3-cyanopyridine.
2 Normative References
The contents of the following documents constitute essential provisions of this document through normative reference. For dated references, only the edition corresponding to the date applies. For undated references, the latest edition (including any amendments) applies.
GB/T 601 Chemical Reagents — Preparations of Standard Volumetric Solutions
GB/T 617 Chemical Reagents — General Method for the Determination of Melting Range
GB/T 6682 Water for Analytical Laboratory Use — Specification and Test Methods
GB/T 8170 Rules of Rounding Off for Numerical Values & Expression and Judgment of Limiting Values
GB/T 9724 Chemical Reagents — General Rules for the Determination of pH
GB 10648 Feed Labeling
GB/T 14699 Feeding Stuffs — Sampling
NY/T 4689 Test for Limit of Heavy Metals in Feed Additives — Turbidimetric Method
3 Terms and Definitions
No terms and definitions are stipulated in this document.
4 Chemical Name, Molecular Formula, Relative Molecular Mass and Structural Formula
4.1 Chemical Name
Pyridine-3-carboxamide.
4.2 Molecular Formula
C₆H₆N₂O.
4.3 Relative Molecular Mass
122.13 (based on the 2022 international relative atomic masses).
4.4 Structural Formula
Shown in Figure 1.
5 Technical Requirements
5.1 Appearance and Properties
Shall be a white crystalline powder or white granular powder, odorless or almost odorless.
5.2 Identification
5.2.1 Chemical identification: When an aqueous solution of the sample is heated under alkaline conditions, the ammonia gas generated should turn moistened red litmus paper blue; after neutralization, upon adding copper sulfate solution, a pale blue precipitate should slowly form.
5.2.2 Ultraviolet identification: An aqueous solution of the sample should have a maximum absorption at a wavelength of (262 ± 2) nm and a minimum absorption at a wavelength of (245 ± 2) nm. The ratio of the absorbance at (245 ± 2) nm to that at (262 ± 2) nm should be 0.63 to 0.67.
5.2.3 Infrared identification: The infrared spectrum of the sample should be consistent with that of the nicotinamide reference standard.
5.3 Physicochemical Indicators
Shall comply with the requirements specified in Table 1.
5.4 Hygiene Indicators
Shall comply with the requirements specified in Table 2.
6 Sampling
Carry out according to GB/T 14699.
7 Test Methods
Warning — Some reagents used in the test methods are corrosive and toxic. Exercise caution during operation and take appropriate safety and protective measures.
7.1 General Provisions
Unless otherwise specified, all reagents used shall be of analytical grade; all standard volumetric solutions shall be prepared and standardized according to GB/T 601; water used in tests shall be Grade 3 water as specified in GB/T 6682.
7.2 Appearance and Properties
Place an appropriate amount of the sample on a clean, dry white porcelain plate. Observe its color and state, and smell its odor under natural light.
7.3 Identification
7.3.1 Reagents or Materials
7.3.1.1 Potassium bromide: spectroscopic grade.
7.3.1.2 Sulfuric acid solution: Measure 57 ml of sulfuric acid, slowly add it to 300 ml of water while stirring (avoid excessive heat generation). After cooling, dilute with water to 1000 ml and mix well.
7.3.1.3 Sodium hydroxide solution: Weigh 4.3 g of sodium hydroxide, dissolve in water to make 100 ml, and mix well.
7.3.1.4 Copper sulfate solution: Weigh 12.5 g of copper sulfate pentahydrate, dissolve in water to make 100 ml, and mix well.
7.3.1.5 Phenolphthalein indicator solution: Weigh 1 g of phenolphthalein, dissolve in 100 ml of ethanol, and dilute to 100 ml with ethanol.
7.3.1.6 Red litmus paper.
7.3.2 Apparatus
7.3.2.1 Ultraviolet-visible spectrophotometer: wavelength accuracy ±2 nm.
7.3.2.2 Infrared spectrophotometer: resolution ≤ 4.00 cm⁻¹.
7.3.2.3 Analytical balance: accuracy 0.1 mg.
7.3.3 Identification Methods
7.3.3.1 Chemical Identification
Weigh approximately 0.1 g of the sample, dissolve in 5 ml of water, add 5 ml of sodium hydroxide solution (7.3.1.3), and heat gently. The ammonia gas generated should turn moistened red litmus paper (7.3.1.6) blue. Continue heating until the ammonia gas is completely expelled, allow to cool, add 1 to 2 drops of phenolphthalein indicator solution
