标准编号:	GB 4789.15-2010
中文名称:	食品微生物学检验 霉菌和酵母计数
英文名称:	Food Microbiological Examination: Enumeration of Moulds and Yeasts
中标分类:	C53    食品卫生
行业分类:	GB    国家标准
发布机构:	卫生部
发布日期:	2010-03-26
实施日期:	2010-6-1
标准状态:	被替代
被新标准替代:	GB 4789.15-2016 食品安全国家标准 食品微生物学检验 霉菌和酵母计数
被替代日期:	2017-4-19
替代旧标准:	GB/T 4789.15-2003 食品卫生微生物学检验 霉菌和酵母计数
翻译语言:	英文
文件格式:	PDF
中文字符数:	4,000 字
翻译价格(元):	100.00 元
交付时间:	付款后 1 个工作日

1 Scope



This standard specifies the enumeration methods of moulds and yeasts in food.

This standard is applicable to the enumeration of moulds and yeasts in various food.



2 Equipments and Materials



Except conventional sterilization in microbiology Lab. and culture equipments, other equipments and materials are listed as follows:

2.1 Refrigerator: 2℃~ 5℃.

2.2 Thermostatic incubator: 28℃±1℃.

2.3 Homogenizer.

2.4 Thermostatic oscillator.

2.5 Microscope: 10×~100×.

2.6 Electronic balance: sensibility 0.1g.

2.7 Sterile conical flask: capacity 500mL, 250mL.

2.8 Sterile jar: 500mL.

2.9 Sterile sucker: 1mL (with 0.01mL scales), 10mL (with 0.1mL scales).

2.10 Sterile plate: diameter 90 mm.

2.11 Sterile test tube: 10mm×75mm.

2.12 Sterile kraft bag and plastic bag.



3 Culture Medium and Reagent



3.1 Potato dextrose agar medium: see A.1 in Annex A.

3.2 Rose Bengal medium: see A.2 in Annex A.

Contents
Foreword
1 Scope
2 Equipments and Materials
3 Culture Medium and Reagent
4 Examination Procedures
5 Operation Steps
6 Results and Reports
Annex A (Normative) Culture Medium and Reagent
Annex B (informative) Direct Microscopy Enumeration Method of Moulds